MULTISENSORS FOR WHOLE-CELL ANALYTICS
Ingo Tobehn-Steinhäuser
itobehn@cismst.deCiS Forschungsinstitut für Mikrosensorik GmbH (Germany)
Margarita Günther
Technische Universität Dresden, Solid-State Electronics Laboratory (Germany)
Stefan Görlandt
CiS Forschungsinstitut für Mikrosensorik GmbH (Germany)
Steffen Herbst
CiS Forschungsinstitut für Mikrosensorik GmbH (Germany)
Heike Wünscher
CiS Forschungsinstitut für Mikrosensorik GmbH (Germany)
Thomas Ortlepp
CiS Forschungsinstitut für Mikrosensorik GmbH (Germany)
Gerald Gerlach
Technische Universität Dresden, Solid-State Electronics Laboratory (Germany)
Abstract
Whole-cell biosensors, which can be used in the environmental protection and process measuring technology, in the biotechnology, food and pharmaceutical industry for the on-line control and monitoring of chemical and biochemical processes and for the fast detection of small amounts of solutes, are of great interest. These kind of biosensors comprise microorganisms like genetically modified yeast cells, which react on a specific stimulus, e.g. a residual amount of drugs in the measurement medium with a dependent fluorescence. The receptors of the cells detect specific signal molecules and stimulate the organism to produce a fluorescent protein. In this work, a combined impedimetric-optical read out was used, which can provide the reliable and long-term stable detection of a fluorescence signal accompanied by impedance measurements monitoring cell vitality and activity. A new challenge thereby is a special sensor design which enables the integration of excitation source, photodetector, interdigitated electrodes, and the fluidic system with an effective and long-term-stable packaging.
Keywords:
Diclofenac, impedance spectroscopy, transparent ITO-electrodes, switchable electrical interdigital fingers, multiplexerReferences
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Authors
Ingo Tobehn-Steinhäuseritobehn@cismst.de
CiS Forschungsinstitut für Mikrosensorik GmbH Germany
Authors
Margarita GüntherTechnische Universität Dresden, Solid-State Electronics Laboratory Germany
Authors
Stefan GörlandtCiS Forschungsinstitut für Mikrosensorik GmbH Germany
Authors
Steffen HerbstCiS Forschungsinstitut für Mikrosensorik GmbH Germany
Authors
Heike WünscherCiS Forschungsinstitut für Mikrosensorik GmbH Germany
Authors
Thomas OrtleppCiS Forschungsinstitut für Mikrosensorik GmbH Germany
Authors
Gerald GerlachTechnische Universität Dresden, Solid-State Electronics Laboratory Germany
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