MULTISENSORS FOR WHOLE-CELL ANALYTICS


Abstract

Whole-cell biosensors, which can be used in the environmental protection and process measuring technology, in the biotechnology, food and pharmaceutical industry for the on-line control and monitoring of chemical and biochemical processes and for the fast detection of small amounts of solutes, are of great interest. These kind of  biosensors comprise microorganisms like genetically modified yeast cells, which react on a specific stimulus, e.g. a residual amount of drugs in the measurement medium with a dependent fluorescence. The receptors of the cells detect specific signal molecules and stimulate the organism to produce a fluorescent protein. In this work, a combined impedimetric-optical read out was used, which can provide the reliable and long-term stable detection of a fluorescence signal accompanied by impedance measurements monitoring cell vitality and activity. A new challenge thereby is a special sensor design which enables the integration of excitation source,  photodetector, interdigitated electrodes, and the fluidic system with an effective and long-term-stable packaging.


Keywords

Diclofenac; impedance spectroscopy; transparent ITO-electrodes; switchable electrical interdigital fingers; multiplexer

Bidoglio G., Contini S., Gawlik B.M., Locoro G., Loos R., Rimaviciute E.: EU-wide survey of polar organic persistent pollutants in European river waters. Environmental Pollution 157/2009, 561–568.

Falås P., Jewell K.S., Joss A., Ternes, T.A. Wick A: Transformation of diclofenac in hybrid biofilm–activated sludge processes. Water Research 105/2016, 559–567.

Günther M. et al.: Kombinierter optisch-impedimetrischer Ganzzellbiosensor, 13. Dresdner Sensor-Symposium, Dresden 2017, 120–125.

Lide D.R.: Handbook of chemistry and physics – 83rd edition. CRC Press LLC, 2002–2003.

Meißner M.: Arzneimittel in der Umwelt: Natur als Medikamentendeponie, Dtsch Arztebl 105(24)/2008, A-1324 / B-1143 / C-1118.

Pliquett U. et. al.: Schlussbericht. IGF-Vorhaben-Nummer 16925 BR/2; Grundlegende Untersuchungen zu Miniaturelektrodenarrays für die Impedanzspektroskopische zelluläre Biosensorik.

Schröder M. et al.: Hochintegrierte Ganzzellsensoren für die Umwelt- und Medizintechnik. 13. Dresdner Sensor-Symposium, Dresden 2017, 148–152.

Download

Published : 2018-05-30


Tobehn-Steinhäuser, I., Günther, M., Görlandt, S., Herbst, S., Wünscher, H., Ortlepp, T., & Gerlach, G. (2018). MULTISENSORS FOR WHOLE-CELL ANALYTICS . Informatyka, Automatyka, Pomiary W Gospodarce I Ochronie Środowiska, 8(2), 39-41. https://doi.org/10.5604/01.3001.0012.0702

Ingo Tobehn-Steinhäuser  itobehn@cismst.de
CiS Forschungsinstitut für Mikrosensorik GmbH  Germany
Margarita Günther 
Technische Universität Dresden, Solid-State Electronics Laboratory  Germany
Stefan Görlandt 
CiS Forschungsinstitut für Mikrosensorik GmbH  Germany
Steffen Herbst 
CiS Forschungsinstitut für Mikrosensorik GmbH  Germany
Heike Wünscher 
CiS Forschungsinstitut für Mikrosensorik GmbH  Germany
Thomas Ortlepp 
CiS Forschungsinstitut für Mikrosensorik GmbH  Germany
Gerald Gerlach 
Technische Universität Dresden, Solid-State Electronics Laboratory  Germany