CONCEPT AND REALIZATION OF BACKPACK-TYPE SYSTEM FOR MULTICHANNEL ELECTROPHYSIOLOGY IN FREELY BEHAVING RODENTS
Technologies for multichannel electrophysiology are experiencing astounding growth. Numbers of channels reach thousands of recording sites, systems are often combined with electrostimulations and optic stimulations. However, the task of design the cheap, flexible system for freely behaving animals without tethered cable are not solved completely. We propose the system for multichannel electrophysiology for both rats and mice. The system allows to record unit activity and local field potential (LFP) up to 32 channels with different types of electrodes. The system was constructed using Intan technologies RHD 2132 chip. Data acquisition and recordings take place on the DAQ-card, which is placed as a back-pack on the animal. The signal is amplified with amplifier cascade and digitalized with 16-bit ADC. Instrumental filters allow to filter the signal in 0.1–20000 Hz bandwidth. The system is powered from the mini-battery with capacity 340 mA/hr. The system was validated with generated signals, in anaesthetized rat and showed a high quality of recordings.
multichannel electrophysiology; freely behaving rodents; unit activity; local field potential
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