REAL-TIME MONITORING OF CELL CULTURES WITH NICKEL COMB CAPACITORS
The aim of the study was to present a method for assessing the condition of cell culture by measuring the impedance of cells cultured in the presence of nickel. For this purpose, an impedance measurement technique using nickel comb capacitors was used. The capacitor electrodes were made using a thin film magnetron sputtering. In the experimental part, the culture of cells of mouse fibroblasts on the prepared substrate was performed. The cell culture lasted 43 hours and showed that the presented technique allows it to be used to analyze the effect of nickel on cells.
BioMEMS; ECIS; nickel; thin films
Arias L.R., Carla A.P., Yang L.: Real-Time Electrical Impedance Detection of Cellular Activities of Oral Cancer Cells. Biosensors and Bioelectronics 25(10), 2010, 2225–2231. DOI: https://doi.org/10.1016/j.bios.2010.02.029
Arndt S., Seebach J., Psathaki K., Galla H.J., Wegener J.: Bioelectrical Impedance Assay to Monitor Changes in Cell Shape during Apoptosis. Biosensors and Bioelectronics 19(6), 2004, 583–594. DOI: https://doi.org/10.1016/S0956-5663(03)00269-0
Bagnaninchi P.O., Drummond N.: Real-Time Label-Free Monitoring of Adipose-Derived Stem Cell Differentiation with Electric Cell-Substrate Impedance Sensing. Proceedings of the National Academy of Sciences of the United States of America 108(16), 2011, 6462–6467. DOI: https://doi.org/10.1073/pnas.1018260108
Giaever I., Keese C.R.: A Morphological Biosensor for Mammalian Cells. Nature 366(6455), 1993, 591–592.
Hong J., Kandasamy K., Marimuthu M., Choi M.S., Kim S.: Electrical Cell-Substrate Impedance Sensing as a Non-Invasive Tool for Cancer Cell Study. The Analyst 136(2), 2011, 237–245. DOI: https://doi.org/10.1039/C0AN00560F
Hug T.S.: Biophysical Methods for Monitoring Cell-Substrate Interactions in Drug Discovery. ASSAY and Drug Development Technologies 1(3), 2003, 479–488. DOI: https://doi.org/10.1089/154065803322163795
Scholten K., Meng E.: Materials for Microfabricated Implantable Devices: A Review. Lab on a Chip 15(22), 2015, 4256–4272. DOI: https://doi.org/10.1039/C5LC00809C
Stensaas S.S., Stensaas L.J.: Histopathological Evaluation of Materials Implanted in the Cerebral Cortex. Acta Neuropathologica 41(2), 1978, 145–155. DOI: https://doi.org/10.1007/BF00689766
Xiao C., Luong J.H.T.: On-Line Monitoring of Cell Growth and Cytotoxicity Using Electric Cell-Substrate Impedance Sensing (ECIS). Biotechnology Progress 19(3), 2003, 1000–1005. DOI: https://doi.org/10.1021/bp025733x
Xu Y., Xie X., Duan Y., Wang L., Cheng Z., Cheng J.: A Review of Impedance Measurements of Whole Cells. Biosensors and Bioelectronics 77(March), 2016, 824–836. DOI: https://doi.org/10.1016/j.bios.2015.10.027
Zudaire E., Cuesta N., Murty V., Woodson K., Adams L., Gonzalez N., Martínez A., Narayan G., Kirsch I., Franklin W., Hirsch F., Birrer M., Cuttitta F.: The Aryl Hydrocarbon Receptor Repressor Is a Putative Tumor Suppressor Gene in Multiple Human Cancers. Journal of Clinical Investigation 118(2), 2008, 640–650. DOI: https://doi.org/10.1172/JCI30024
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.