REAL-TIME MONITORING OF CELL CULTURES WITH NICKEL COMB CAPACITORS

Andrzej Kociubiński

akociub@o2.plsemiconductor.pl
Lublin Univeristy of Technology, Department of Electronics and Computer Science (Poland)
http://orcid.org/0000-0002-0377-8243

Dawid Zarzeczny


Lublin Univeristy of Technology, Department of Electronics and Computer Science (Poland)
http://orcid.org/0000-0003-2029-9962

Maciej Szypulski


Lublin Univeristy of Technology, Department of Electronics and Computer Science (Poland)
http://orcid.org/0000-0002-6227-3624

Aleksandra Wilczyńska


Lublin Univeristy of Technology, Department of Electronics and Computer Science (Poland)
http://orcid.org/0000-0002-5630-1078

Dominika Pigoń


Maria Curie-Skłodowska University in Lublin (Poland)
http://orcid.org/0000-0002-7545-3237

Teresa Małecka-Massalska


Medical University of Lublin (Poland)
http://orcid.org/0000-0003-3384-0324

Monika Prendecka


Medical University of Lublin (Poland)
http://orcid.org/0000-0001-9414-4344

Abstract

The aim of the study was to present a method for assessing the condition of cell culture by measuring the impedance of cells cultured in the presence of nickel. For this purpose, an impedance measurement technique using nickel comb capacitors was used. The capacitor electrodes were made using a thin film magnetron sputtering. In the experimental part, the culture of cells of mouse fibroblasts on the prepared substrate was performed. The cell culture lasted 43 hours and showed that the presented technique allows it to be used to analyze the effect of nickel on cells.


Keywords:

BioMEMS, ECIS, nickel, thin films

Arias L.R., Carla A.P., Yang L.: Real-Time Electrical Impedance Detection of Cellular Activities of Oral Cancer Cells. Biosensors and Bioelectronics 25(10), 2010, 2225–2231.
DOI: https://doi.org/10.1016/j.bios.2010.02.029   Google Scholar

Arndt S., Seebach J., Psathaki K., Galla H.J., Wegener J.: Bioelectrical Impedance Assay to Monitor Changes in Cell Shape during Apoptosis. Biosensors and Bioelectronics 19(6), 2004, 583–594.
DOI: https://doi.org/10.1016/S0956-5663(03)00269-0   Google Scholar

Bagnaninchi P.O., Drummond N.: Real-Time Label-Free Monitoring of Adipose-Derived Stem Cell Differentiation with Electric Cell-Substrate Impedance Sensing. Proceedings of the National Academy of Sciences of the United States of America 108(16), 2011, 6462–6467.
DOI: https://doi.org/10.1073/pnas.1018260108   Google Scholar

Giaever I., Keese C.R.: A Morphological Biosensor for Mammalian Cells. Nature 366(6455), 1993, 591–592.
  Google Scholar

Hong J., Kandasamy K., Marimuthu M., Choi M.S., Kim S.: Electrical Cell-Substrate Impedance Sensing as a Non-Invasive Tool for Cancer Cell Study. The Analyst 136(2), 2011, 237–245.
DOI: https://doi.org/10.1039/C0AN00560F   Google Scholar

Hug T.S.: Biophysical Methods for Monitoring Cell-Substrate Interactions in Drug Discovery. ASSAY and Drug Development Technologies 1(3), 2003, 479–488.
DOI: https://doi.org/10.1089/154065803322163795   Google Scholar

Scholten K., Meng E.: Materials for Microfabricated Implantable Devices: A Review. Lab on a Chip 15(22), 2015, 4256–4272.
DOI: https://doi.org/10.1039/C5LC00809C   Google Scholar

Stensaas S.S., Stensaas L.J.: Histopathological Evaluation of Materials Implanted in the Cerebral Cortex. Acta Neuropathologica 41(2), 1978, 145–155.
DOI: https://doi.org/10.1007/BF00689766   Google Scholar

Xiao C., Luong J.H.T.: On-Line Monitoring of Cell Growth and Cytotoxicity Using Electric Cell-Substrate Impedance Sensing (ECIS). Biotechnology Progress 19(3), 2003, 1000–1005.
DOI: https://doi.org/10.1021/bp025733x   Google Scholar

Xu Y., Xie X., Duan Y., Wang L., Cheng Z., Cheng J.: A Review of Impedance Measurements of Whole Cells. Biosensors and Bioelectronics 77(March), 2016, 824–836.
DOI: https://doi.org/10.1016/j.bios.2015.10.027   Google Scholar

Zudaire E., Cuesta N., Murty V., Woodson K., Adams L., Gonzalez N., Martínez A., Narayan G., Kirsch I., Franklin W., Hirsch F., Birrer M., Cuttitta F.: The Aryl Hydrocarbon Receptor Repressor Is a Putative Tumor Suppressor Gene in Multiple Human Cancers. Journal of Clinical Investigation 118(2), 2008, 640–650.
DOI: https://doi.org/10.1172/JCI30024   Google Scholar

Download


Published
2020-06-30

Cited by

Kociubiński, A., Zarzeczny, D., Szypulski, M., Wilczyńska, A., Pigoń, D., Małecka-Massalska, T., & Prendecka, M. (2020). REAL-TIME MONITORING OF CELL CULTURES WITH NICKEL COMB CAPACITORS. Informatyka, Automatyka, Pomiary W Gospodarce I Ochronie Środowiska, 10(2), 32–35. https://doi.org/10.35784/iapgos.1564

Authors

Andrzej Kociubiński 
akociub@o2.plsemiconductor.pl
Lublin Univeristy of Technology, Department of Electronics and Computer Science Poland
http://orcid.org/0000-0002-0377-8243

Authors

Dawid Zarzeczny 

Lublin Univeristy of Technology, Department of Electronics and Computer Science Poland
http://orcid.org/0000-0003-2029-9962

Authors

Maciej Szypulski 

Lublin Univeristy of Technology, Department of Electronics and Computer Science Poland
http://orcid.org/0000-0002-6227-3624

Authors

Aleksandra Wilczyńska 

Lublin Univeristy of Technology, Department of Electronics and Computer Science Poland
http://orcid.org/0000-0002-5630-1078

Authors

Dominika Pigoń 

Maria Curie-Skłodowska University in Lublin Poland
http://orcid.org/0000-0002-7545-3237

Authors

Teresa Małecka-Massalska 

Medical University of Lublin Poland
http://orcid.org/0000-0003-3384-0324

Authors

Monika Prendecka 

Medical University of Lublin Poland
http://orcid.org/0000-0001-9414-4344

Statistics

Abstract views: 524
PDF downloads: 334